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Article # : SC08

Sema Çalış, Ph.D.

Professor

Evaluation of the irritation of a new potent antimicrobial surfactant, C31G : an irrigation study on albino rabbit eyes

N. Ünlü, S. Çalış, M. İrkeç, F. Aksoy, M. Orhan, M. Şumnu and A.A. Hıncal
 

Hacettepe University, Ankara
Numune Hospital, Ankara

Summary

C31G is a specific formulation which contains equal molar concentrations of alkyi N-betaine and alkyi N-N dimethylamine oxide. This amphoteric surface active agent demonstrates a broad spectrum of antimicrobial effectiveness. C31G has a bactericidal effect on Pseudomonas aeruginosa and is non-irritant to the rabbit eye as has been shown by the Draize test. The purpose of the present study is to investigate the cytotoxiciry of C31G at minimum bactericidal concentrations in the rabbit eye. Isotonic and sterile solutions of C31G were prepared at 0.02% concentration in Sorensen phosphate buffer (pH 7.4). The right eyes of 12 albino rabbits were irrigated wich 400 ml of these test solutions. Left eyes served as the control. After an exposure time of 1 h, each animal was killed. The eyes, after being enucleated, were kept in formaldehyde solution. 5 µm sections were stained with hematoxylene-eosin and examined by an optical microscope for possible cellular damage. The present work showed that. among the experimental group, 5 animals had conjunctivitis and 2 had keratoconjunctivitis. In 2 animals of the control group, there was a slight infiltration of lymphocytes into the ciliary body. No severe inflammation was determined in either group. It was concluded that C31G was a mild irritant to the rabbit eye during an exposure time of  1 h.

Key words

Antimicrobial surfactant,  Ocular irritation,  In vivo assessment.