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Article # : SC33

Sema Çalış, Ph.D.

Professor

In vitro/in vivo evaluation of the efficiency of

teicoplanin-loaded biodegradable microparticles

formulated for implantation to infected bone defects

İ.YENİCE†,   S. ÇALIŞ †*,  B. ATİLLA‡,  H.  S. KAކ,  M. ÖZALP†, M.EKİZOĞLU‡, A. BİLGİLݧ  and    A.  A. HINCAL†

† Hacettepe University, Faculty of Pharmacy, Department of  Pharmaceutical Technology

‡ Faculty of Medicine, Department of Orthopaedics and Traumatology, 06100  Sihhiye-Ankara,

   Turkey

§ Ankara University,Veterinary Faculty,Department of Orthopaedics and Traumatology, Ankara, 

   Turkey

Summary

Chronic osteomyelitis is still the cause of many problems in orthopaedics in terms of therapy and infection persistence. Four-to-six week systemic antibiotic therapy is required along with bone and soft tissue debridement in the therapy of chronic osteomyelitis. Prolonged-release local antibiotic therapy has been taken into consideration due to the side e .ects encountered in long-term high dose antibiotic use and the duration of hospitalization of the patients. Although local antibiotic therapy has been achieved by bone cement,a second surgical operation is needed for the removal of the system. On the other hand,heat generation during cement curing limits the use of heat-sensitive active ingredients. The most frequent osteomyelitis inducing micro-organism is gram (+) Staphylococcus aureus .In this study, teicoplanin,a glycopeptide antibiotic, active on gram (+) bacteria, was incorporated in a synthetic polymer in order to prepare a microsphere formulation for implantation to bone defects. Particle size, surface characteristics, loading capacity and in vitro release characteristics of the microspheres were determined as well as stability assessment of  teicoplanin under accelerated conditions.In vivo studies were performed on rabbits and the microparticles were implanted intra-articularly to the lateral condylus of the femur.Antibiotic presence was detected by a microbiological assay from synovial .uid sample aspirated throughout 5 weeks. In the light of these evaluations, microspheres prepared from PLGA (75:25) (Mw 136 000) polymer were determined to be effective, and promising for obtaining prolonged local antibiotic release.

Key words

Implant,PLGA microspheres,teicoplanin,microbiological assay, osteomyelitis.